Determination of serum total iron-binding capacity.
نویسندگان
چکیده
منابع مشابه
Simplified determination of serum iron and total iron-binding capacity.
2,4,6-Tripyridyl-s-triazine (TPTZ) is a color reagent for ferrous ion in the pH range 2 to 10,when ascorbic acid is used as the reducing agent. In this range TPTZ and ferrous ion combine in the ratio of two to one. Tris buffer is commonly used to maintain an alkaline pH, but it reacts with other reducing agents, rendering them ineffective. These observations suggested a method for the joint det...
متن کاملDetermination of the Serum Iron-binding Capacity.
A colorimetric and an isotopic method for determining the iron-binding capacity are described, using only 1 ml. samples of plasma or serum. Both methods are simple, rapid and sensitive. Satisfactory results are obtained from frozen as well as from fresh samples.
متن کامل[Determination of serum iron and iron-binding capacity].
Rapid methods depending on differential absorptiometry are described for the determination of the transferrin iron content and the latent iron-binding capacity of blood serum. Each determination requires as little as 0.5 ml. serum. The methods are well adapted for routine use in the ;average' laboratory. Three or four sera may be completely analysed in 30 minutes. All operations are carried out...
متن کاملImproved direct specific determination of serum iron and total iron-binding capacity.
Serum iron is released from transferrin and reduced at pH 1.7 by treating serum with a 10 g/L ascorbic acid solution in 0.1 mol/L HCl. When ferrozine is added to this reagent, it forms a complex with iron that is as intensely colored as at higher pH values, and under these conditions no turbidity is produced. The second major interference, that from copper, is eliminated by adding 1 g of thiose...
متن کاملSerum iron and total iron-binding capacity as measured radiometrically.
With the increasing availability of programmable calculators and minicomputers, it is desirable to demystify radioimmunoassay calculations, and make them available to laboratories lacking massive computing facilities. Recently I modified the Hewlett-Packard 67/97 logit-log radioimmunoassay program to include weighting (submitted to the HP Program Library). I provide here a description of this p...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1973
ISSN: 0021-9746
DOI: 10.1136/jcp.26.6.457-a